Active Recombinant Human GZMM protein, His-tagged

• Cat.No.: GZMM-98H
• Product Overview: Recombinant Human GZMM protein(Ile26~Ala257), fused with N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose
• Bio-activity: GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets α-Tubulin and disorganizes the microtubule network, besides, Ezrin has also been identified as a substrate of GZMM. Therefore, a catalytic assay was conducted to detect the protease activity of recombinant human GZMM using Hela cells lysates. Briefly, protein lysates were extracted from 2×107 Hela cells using Lysis Buffer, then incubated with normal or inactivated GZMM in 37°C for 4h. Samples were immunoblotted using Abs β-actin as control, and Ezrin to detect the enzyme activity. The results were shown below. It is obvious that recombinant human GZMM cleaved Ezrin.
• Molecular Mass: 26.3kDa
• Protein length: Ile26~Ala257
• AA Sequence: MGHHHHHHSGSEF-IIGGR EVIPHSRPYM ASLQRNGSHL CGGVLVHPKW VLTAAHCLAQ RMAQLRLVLG LHTLDSPGLT FHIKAAIQHP RYKPVPALEN DLALLQLDGK VKPSRTIRPL ALPSKRQVVA AGTRCSMAGW GLTHQGGRLS RVLRELDLQV LDTRMCNNSR FWNGSLSPSM VCLAADSKDQ APCKGDSGGP LVCGKGRVLA RVLSFSSRVC TDIFKPPVAT AVAPYVSWIR KVTGRSA
• Purity: >92%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vorte.

Gene Information

• Gene Name: GZMM granzyme M (lymphocyte met-ase 1) [ Homo sapiens ]
• Official Symbol: GZMM
• Synonyms: GZMM; granzyme M (lymphocyte met-ase 1); granzyme M; LMET1; lymphocyte met ase 1; MET1; met-ase; HU-Met-1; lymphocyte met-ase 1; Met-1 serine protease; natural killer cell granular protease
• Gene ID: 3004
• mRNA Refseq: NM_005317
• Protein Refseq: NP_005308
• MIM: 600311
• UniProt ID: P51124