Active Recombinant Human GUSB Protein

• Cat.No.: GUSB-001H
• Product Overview: Recombinant human GUSB protein containing 635 amino acids (23-651a.a) fused to a 6 amino acid His-tag at C-terminus was produced in Sf9 Baculovirus cells.

Specification

• Species: Human
• Source: Baculovirus
• Tag: His
• Protein Length: 23-651 a.a.
• Description: This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII.
• Form: Sterile Filtered colorless solution
• Bio-activity: Specific activity is > 1600 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-Methylumbelliferone to 4- Methylum-belliferyl-β-D-glucosiduronic acid per minute at 37 centigrade and pH6.0.
• Molecular Mass: 73.4 kDa
• AA Sequence: LQGGMLYPQESPSRECKELDGLWSFRADFSDNRRRGFEEQWYRRPLWESGPTVDMPVPSSFNDISQDWRLRHFVGWVWYEREVILPERWTQDLRTRVVLRIGSAHSYAIVWVNGVDTLEHEGGYLPFEADISNLVQVGPLPSRLRITIAINNTLTPTTLPPGTIQYLTDTSKYPKGYFVQNTYFDFFNYAGLQRSVLLYTTPTTYIDDITVTTSVEQDSGLVNYQISVKGSNLFKLEVRLLDAENKVVANGTGTQGQLKVPGVSLWWPYLMHERPAYLYSLEVQLTAQTSLGPVSDFYTLPVGIRTVAVTKSQFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNLLRWLGANAFRTSHYPYAEEVMQMCDRYGIVVIDECPGVGLALPQFFNNVSLHHHMQVMEEVVRRDKNHPAVVMWSVANEPASHLESAGYYLKMVIAHTKSLDPSRPVTFVSNSNYAADKGAPYVDVICLNSYYSWYHDYGHLELIQLQLATQFENWYKKYQKPIIQSEYGAETIAGFHQDPPLMFTEEYQKSLLEQYHLGLDQKRRKYVVGELIWNFADFMTEQSPTRVLGNKKGIFTRQRQPKSAAFLLRERYWKIANETRYPHSVAKSQCLENSLFTHHHHHH
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Usage: This products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
• Storage: Store at 4 centigrade if entire vial will be used within 2-4 weeks. Store, frozen at -20 centigrade for longer periods of time.
  For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  Avoid multiple freeze-thaw cycles.
• Storage Buffer: GUSB protein solution (0.25 mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

Gene Information

• Gene Name: GUSB glucuronidase beta [ Homo sapiens (human) ]
• Official Symbol: GUSB
• Synonyms: GUSB; glucuronidase beta; BG; MPS7; beta-glucuronidase; beta-D-glucuronidase; beta-G1; EC 3.2.1.31
• Gene ID: 2990
• mRNA Refseq: NM_000181
• Protein Refseq: NP_000172
• MIM: 611499
• UniProt ID: P08236