Active Recombinant Human GUCY2C protein, His-Avi-tagged, Biotinylated

• Cat.No.: GUCY2C-1499H
• Product Overview: Biotinylated Recombinant Human GUCY2C protein(Ser24-Gln430), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 24-430 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Immobilized Biotinylated Human GUCY2C, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human GUCY2C Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL.Immobilized Monoclonal Anti-Human GUCY2C Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human GUCY2C, His,Avitag with a linear range of 0.2-6 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 49.6 kDa. The protein migrates as 65-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ser24-Gln430
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GUCY2C guanylate cyclase 2C (heat stable enterotoxin receptor) [ Homo sapiens ]
• Official Symbol: GUCY2C
• Synonyms: GUCY2C; guanylate cyclase 2C (heat stable enterotoxin receptor); GUC2C; heat-stable enterotoxin receptor; STAR; GC-C; hSTAR; STA receptor; guanylyl cyclase C; intestinal guanylate cyclase
• Gene ID: 2984
• mRNA Refseq: NM_004963
• Protein Refseq: NP_004954
• MIM: 601330
• UniProt ID: P25092