Active Recombinant Human GSK3 alpha, GST-tagged

• Cat.No.: GSK3A-1411H
• Product Overview: Recombinant full-length human GSK3alpha was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: Full length
• Description: GSK3α is a multifunctional protein serine kinase, homologous to Drosophila shaggy (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase and transcription factors (e.g., JUN). GSK3α also plays a role in the WNT and PI3K signaling pathways. Alzheimer disease is associated with increased production and aggregation of amyloid-beta-40 and -42 peptides into plaques. GSK3α is required for maximal production of the beta-amyloid-40 and -42 peptides generated from the amyloid precursor protein by presenilin-dependent gamma-secretase cleavage. In vitro, lithium, a GSK3α?inhibitor, blocks the production of the beta-amyloid peptides by interfering with the gamma-secretase step.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 184 nmol/min/mg
• Molecular Mass: ~81 kDa
• Purity: >90%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: GSK3A glycogen synthase kinase 3 alpha [ Homo sapiens ]
• Official Symbol: GSK3A
• Synonyms: GSK3A; glycogen synthase kinase 3 alpha; glycogen synthase kinase-3 alpha; GSK-3 alpha; serine/threonine-protein kinase GSK3A; DKFZp686D0638
• Gene ID: 2931
• mRNA Refseq: NM_019884
• Protein Refseq: NP_063937
• MIM: 606784
• UniProt ID: P49840
• Chromosome Location: 19q13
• Pathway: AKT phosphorylates targets in the cytosol, organism-specific biosystem; Activation of Chaperone Genes by XBP1(S), organism-specific biosystem; Activation of Chaperones by IRE1alpha, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein kinase A catalytic subunit binding; protein serine/threonine kinase activity; tau-protein kinase activity