Active Recombinant Human GREM1 Protein, His-tagged

• Cat.No.: GREM1-1004H
• Product Overview: Recombinant Human GREM1 (Accession # O60565) Lys25-Asp184, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: Lys25-Asp184
• Predicted N Terminal: Lys25
• Form: Lyophilized from a 0.2 µm filtered solution in PBS and EDTA.
• Bio-activity: Measured by its ability to inhibit proliferation in MC3T3-E1 mouse preosteoblast cells.The ED50 for this effect is typically 0.025-0.15 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4.
• Molecular Mass: Recombinant Human GREM1, His-tagged has a calculated MW of 19.7 kDa. In SDS-PAGE migrates as 25-30 kDa, under reducing conditions.
• Purity: >90%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: GREM1 gremlin 1 [ Homo sapiens ]
• Official Symbol: GREM1
• Synonyms: GREM1; gremlin 1; CKTSF1B1, cysteine knot superfamily 1, BMP antagonist 1 , gremlin 1, cysteine knot superfamily, homolog (Xenopus laevis); gremlin-1; DAND2; DRM; gremlin; gremlin 1-like protein; DAN domain family member 2; increased in high glucose-2; proliferation-inducing gene 2; increased in high glucose protein 2; cell proliferation-inducing gene 2 protein; cysteine knot superfamily 1, BMP antagonist 1; gremlin 1, cysteine knot superfamily, homolog; down-regulated in Mos-transformed cells protein; PIG2; IHG-2; GREMLIN; CKTSF1B1; MGC126660
• Gene ID: 26585
• mRNA Refseq: NM_001191322
• Protein Refseq: NP_001178251
• MIM: 603054
• UniProt ID: O60565
• Chromosome Location: 15q13.3