Active Recombinant Human GPI Protein, His-tagged

• Cat.No.: GPI-387H
• Product Overview: Recombinant human GPI, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Glucose-6-phosphate isomerase, also known as GPI, belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
• Form: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol.
• Bio-activity: Specific activity is >400 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will convert 1.0 umole of D-Fructose 6-phosphate to D-glucose 6-phosphate per minute at pH 7.4 at 37C.
• Molecular Mass: 65.3kDa (578aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MAALTRDPQF QKLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH VALRNRSNTP ILVDGKDVMP EVNKVLDKMK SFCQRVRSGD WKGYTGKTIT DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA HWMDQHFRTT PLEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK QQREARVQ
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: GPI glucose-6-phosphate isomerase [ Homo sapiens ]
• Official Symbol: GPI
• Synonyms: GPI; glucose-6-phosphate isomerase; glucose phosphate isomerase; AMF; NLK; neuroleukin; oxoisomerase; sperm antigen 36; sperm antigen-36; phosphohexomutase; phosphosaccharomutase; phosphohexose isomerase; phosphoglucose isomerase; autocrine motility factor; hexosephosphate isomerase; hexose monophosphate isomerase; PGI; PHI; GNPI; SA36; SA-36; DKFZp686C13233
• Gene ID: 2821
• mRNA Refseq: NM_000175
• Protein Refseq: NP_000166
• MIM: 172400
• UniProt ID: P06744
• Chromosome Location: 19q13.1
• Pathway: Amino sugar and nucleotide sugar metabolism, organism-specific biosystem; Amino sugar and nucleotide sugar metabolism, conserved biosystem; Gluconeogenesis, organism-specific biosystem; Glucose metabolism, organism-specific biosystem; Glycolysis, organism-specific biosystem; Glycolysis (Embden-Meyerhof pathway), glucose =>pyruvate, organism-specific biosystem
• Function: cytokine activity; glucose-6-phosphate isomerase activity; glucose-6-phosphate isomerase activity; growth factor activity; isomerase activity