Active Recombinant Human GP1BA Protein, C-His-tagged

• Cat.No.: GP1BA-12H
• Product Overview: Recombinant Human GP1BA Protein, fused to His-tag at C-terminus, was expressed in NS0.

Specification

• Species: Human
• Source: NS0
• Tag: His
• ProteinLength: His17-Leu505
• Description: Glycoprotein Ib (GP Ib) is a platelet surface membrane glycoprotein composed of a heterodimer, an alpha chain and a beta chain, that is linked by disulfide bonds. The Gp Ib functions as a receptor for von Willebrand factor (VWF). The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX and platelet glycoprotein V. The binding of the GP Ib-IX-V complex to VWF facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. This gene encodes the alpha subunit. Mutations in this gene result in Bernard-Soulier syndromes and platelet-type von Willebrand disease. The coding region of this gene is known to contain a polymophic variable number tandem repeat (VNTR) domain that is associated with susceptibility to nonarteritic anterior ischemic optic neuropathy.
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA. Recombinant Human CD42b/GPIb alpha is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human vWF-A2 that produces 50% optimal binding response is approximately 6-36 ng/mL in the presence of ristocetin.
• Molecular Mass: Predicted Molecular Mass: 54.7 kDa
  SDS-PAGE: 95-120 kDa, reducing conditions
• N-terminal Sequence Analysis: His17
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Related Research Areas: Coagulation
  Myeloid Lineage Markers
  Platelet Adhesion Molecules
  Platelet Markers

Gene Information

• Gene Name: GP1BA glycoprotein Ib platelet subunit alpha [ Homo sapiens (human) ]
• Official Symbol: GP1BA
• Synonyms: BSS; GP1B; VWDP; CD42B; GPIbA; BDPLT1; BDPLT3; DBPLT3; GPIbalpha; CD42b-alpha
• Gene ID: 2811
• mRNA Refseq: NM_000173.7
• Protein Refseq: NP_000164.5
• MIM: 606672
• UniProt ID: P07359