Active Recombinant Human GM-CSF

• Cat.No.: CSF3-4H
• Product Overview: Recombinant Human GM-CSF protein, expressed in Pichia, with N Terminal Met-Phe-Asn-Leu-Pro Pichia Pastoris

Specification

• Species: Human
• Source: P.pastoris
• Tag: Non
• Description: GMCSF is a cytokine that controls the production, differentiation, and function of granul centigradeytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been l centigradealized to a cluster of related genes at chromosome region 5q31, which is known to be ass centigradeiated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granul centigradeytes, macrophages, eosinophils and erythr centigradeytes
• Predicted N Terminal: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser.
• Form: The protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM phosphate buffer pH 7.0, 40 mg mannitol and 10 mg sucrose.
• Bio-activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is< 0.183="" ng/ml,="" corresponding="" to="" a="" specific="" activity="" of="" 5="" x="" 106="">
• Molecular Mass: Granul centigradeyte Macrophage Colony Stimulating Factor Human Recombinant produced in Yeast is a single, glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 26-32 kDa. rhGMCSF differs from the natural human GM-CSF by a substitution of leucine at position 23 (R to L), and the carbohydrate moiety may be different from the native protein. GM-CSF is purified by proprietary chromatographic techniques.
• Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis bySDS-PAGE.
• Usage: FOR RESEARCH USE ONLY
• Quality Control Test: Lyophilized Granul centigradeyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution GMCSF should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
• Concentration: 1mg/ml
• Reconstitution: It is recommended to reconstitute the lyophilized Granul centigradeyte Macrophage Colony Stimulating Factor in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
• Warning: Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: CSF3 colony stimulating factor 3 (granulocyte) [ Homo sapiens ]
• Official Symbol: CSF3
• Synonyms: CSF3; colony stimulating factor 3 (granulocyte); C17orf33, chromosome 17 open reading frame 33, G CSF, GCSF; granulocyte colony-stimulating factor; filgrastim; granulocyte colony stimulating factor; lenograstim; MGC45931; pluripoietin; GCSF; CSF3OS; C17orf33
• Gene ID: 1440
• mRNA Refseq: NM_000759
• Protein Refseq: NP_000750
• MIM: 138970
• UniProt ID: P09919
• Chromosome Location: 17q11.2-q12
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem
• Function: cytokine activity; cytokine activity; enzyme binding; granulocyte colony-stimulating factor receptor binding; growth factor activity