Active Recombinant Human GLB1, His-tagged

• Cat.No.: GLB1-7790H
• Product Overview: Recombinant Human GLB1 (Met1-Val677) fused with 6­His tag at C­terminal, was expressed in CHO Cells.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 1-677 a.a.
• Description: This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the terminal beta-galactose from ganglioside substrates and other glycoconjugates. Defects in this gene are the cause of GM1-gangliosidosis and Morquio B syndrome. Multiple transcript variants encoding different isoforms have been found for this gene.
• Predicted N Terminal: Leu24
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl-beta -D-galactopyranoside. The specific activity is >1,800 pmol/min/µg.
• Molecular Mass: 74 kDa
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Stability: Avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: GLB1 galactosidase, beta 1 [ Homo sapiens (human) ]
• Official Symbol: GLB1
• Synonyms: GLB1; EBP; ELNR1; MPS4B; galactosidase, beta 1; beta-galactosidase; lactase; acid beta-galactosidase; elastin receptor 1, 67kDa; EC 3.2.1.23
• Gene ID: 2720
• mRNA Refseq: NM_000404
• Protein Refseq: NP_000395
• MIM: 611458
• UniProt ID: P16278
• Chromosome Location: 3p21.33
• Pathway: Galactose metabolism; Glycosaminoglycan degradation; HS-GAG degradation
• Function: beta-galactosidase activity; galactoside binding; protein binding