Active Recombinant Human GH1 (27-217aa)

• Cat.No.: GH1-240H
• Product Overview: Human GH1 (NP_000506, 27 a.a.-217 a.a.) partial recombinant protein expressed in Escherichia coli.

Specification

• Species: Human
• Source: Human
• Tag: Non
• Description: Somatotropin is a protein that in humans is encoded by the GH1 gene. The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones that play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation, an arrangement thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.
• Sequence: FPTIPLSRL FDNAMLRAHR LHQLAFDTYQ EFEEAYIPKE QKYSFLQNPQ TSLCFSESIP TPSNREETQQ KSNLELLRIS LLLIQSWLEP VQFLRSVFAN SLVYGASDSN VYDLLKDLEE GIQTLMGRLE DGSPRTGQIF KQTYSKFDTN SHNDDALLKN YGLLYCFRKD MDKVETFLRI VQCRSVEGSC GF
• Theoretical MW (kDa): 22.2
• Form: Liquid
• Preparation Method: Escherichia coli expression system
• Purity: > 95% by SDS-PAGE
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Activity: The proliferative activity of rhGH was tested in culture using rat Nb2 Lymphoma cells
• Application: SDS-PAGE
• Storage Buffer: In 88 mM mannitol, 5 mM pH ospH ate, pH 7.4
• Storage: Store at 4°C for 1-2 weeks. For long term storage store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.
• Unitprot ID: P01241
• Pathways: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Neuroactive ligand-receptor interaction

Gene Information

• Gene Name: GH1 growth hormone 1 [ Homo sapiens ]
• Official Symbol: GH1
• Synonyms: GH; GH-N; GHN; hGH-N; Growth hormone; Pituitary growth hormone; growth hormone 1; GH1; IGHD1B; Somatotropin; hGH
• Gene ID: 2688
• mRNA Refseq: NM_000515
• Protein Refseq: NP_000506
• MIM: 139250
• Chromosome Location: 17q24.2
• Function: growth factor activity; growth hormone activity; growth hormone receptor binding; prolactin receptor binding; protein binding