Active Recombinant Human GFAP protein, His-tagged

• Cat.No.: GFAP-468H
• Product Overview: Recombinant Human GFAP protein(Met 1 - Met 432), fused with His tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 500 mM NaCl, 0.5 M Arginine, pH8.0 with trehalose as protectant.
• Bio-activity: Immobilized Human GFAP, His Tag at 1 μg/mL (100 μL/well) can bind GFAP Monoclonal Antibody with a linear range of 0.8-25 ng/mL.
• Molecular Mass: The protein has a calculated MW of 51.9 kDa. The protein migrates as 50 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: Met 1 - Met 432
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: GFAP glial fibrillary acidic protein [ Homo sapiens ]
• Official Symbol: GFAP
• Synonyms: GFAP; glial fibrillary acidic protein; FLJ45472; intermediate filament protein; FLJ42474
• Gene ID: 2670
• mRNA Refseq: NM_001131019
• Protein Refseq: NP_001124491
• MIM: 137780
• UniProt ID: P14136