Active Recombinant Human GDF6 Protein (Thr336-Arg455), C-His tagged, Animal-free, Carrier-free

• Cat.No.: GDF6-188H
• Product Overview: Recombinant Human GDF6 Protein (Thr336-Arg455) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Bone Morphogenetic Protein-13 (BMP-13), known as Growth differentiation factor 6 (GDF6), is an extracellular multifunctional cytokine that is also a member of the TGFβ family. BMP-13 can bind with the TGFβ receptor and induce SMAD protein signal transduction. BMP-13 is a regulatory protein that can affect the growth and differentiation of developing embryos. Moreover, it plays a role in regulating the patterning of the ectoderm and controlling eye development.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is 63-240 ng/mL.
• Molecular Mass: The protein has a calculated MW of 14.50 kDa.
  The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Thr336-Arg455
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 2 weeks under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 3.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: GDF6 growth differentiation factor 6 [ Homo sapiens (human) ]
• Official Symbol: GDF6
• Synonyms: GDF6; growth differentiation factor 6; growth/differentiation factor 6; BMP13; GDF-6; Klippel-Feil syndrome; Klip-Feil malformation; Klippel-Feil malformation; growth/differentiation factor 16; KFM; KFS; KFS1; KFSL; SGM1; CDMP2; MCOP4; SCDO4; MCOPCB6; MGC158100; MGC158101
• Gene ID: 392255
• mRNA Refseq: NM_001001557
• Protein Refseq: NP_001001557
• MIM: 601147
• UniProt ID: Q6KF10