Active Recombinant Human GDF2 Protein (Ser320-Arg429), N-His tagged, Animal-free, Carrier-free

• Cat.No.: GDF2-119H
• Product Overview: Recombinant Human GDF2 Protein (Ser320-Arg429) with N-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ser320-Arg429
• Description: Bone Morphogenetic Protein-9 (BMP-9), known as Growth differentiation factor 2 (GDF2), is an extracellular multifunctional cytokine that is also a member of the TGF-β family. BMP-8B can bind with the TGF-β receptor and trigger SMAD protein signal transduction. BMP-9 is the most effective for the differentiation of osteoblasts in vivo, and the general BMP blocker does not affect it.
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is < 0.4 ng/mL.
• Molecular Mass: The protein has a calculated MW of 12.89 kDa.
  The protein migrates as 16 kDa under reducing condition (SDS-PAGE analysis).
• Endotoxin: < 0.01 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 3.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: GDF2 growth differentiation factor 2 [ Homo sapiens (human) ]
• Official Symbol: GDF2
• Synonyms: GDF2; growth differentiation factor 2; growth/differentiation factor 2; BMP 9; BMP9; GDF-2; bone morphogenetic protein 9; BMP-9
• Gene ID: 2658
• mRNA Refseq: NM_016204
• Protein Refseq: NP_057288
• MIM: 605120
• UniProt ID: Q9UK05