Active Recombinant Human GALC, His-tagged

• Cat.No.: GALC-605H
• Product Overview: Recombinant Human GALC (Accession # NP_000144) Met1-Arg685, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Human
• Tag: His
• Predicted N Terminal: Tyr43
• Form: Supplied as a 0.2 µm filtered solution in MES, NaCl and Glycerol.
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl-beta -D-galactopyranoside.The specific activity is >550 pmol/min/ug, as measured under the described conditions.
• Molecular Mass: Recombinant Human GALC, His-tagged has a calculated MW of 74 kDa. In SDS-PAGE migrates as 70-85 kDa, reducing conditions.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Protein length: 1-685 a.a.

Gene Information

• Gene Name: GALC galactosylceramidase [ Homo sapiens ]
• Official Symbol: GALC
• Synonyms: GALC; galactosylceramidase; galactosylceramidase (Krabbe disease); galactocerebrosidase; Krabbe disease; GALCERase; galactosylceraminidase; galactocerebroside beta-galactosidase; galactosylceramide beta-galactosidase
• Gene ID: 2581
• mRNA Refseq: NM_000153
• Protein Refseq: NP_000144
• MIM: 606890
• UniProt ID: P54803
• Chromosome Location: 14q31