Active Recombinant Human GABARAP Protein, His-tagged Protein, Fluorescein

• Cat.No.: GABARAP-580H
• Product Overview: Recombinant Human GABARAP (Accession # NP_009209), fused with an N-terminal 6-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: X mg/ml (XµM) in 50mM Hepes pH 7.5, 100mM NaCl.Actual concentration varies with lot number.
• Bio-activity: Recombinant Human His6-GABARAP/Apg8p1 Fluorescein is ideal for use in assays requiring fluorescent detection. Optimal fluorescence at pH 8.0 is monitored with an excitation wavelength of 490 nM and an emission wavelength of 515 nM. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-GABARAP/Apg8p1 Fluorescein concentration of 0.1-1 µM.
• Purity: >95%, by PAGE.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: GABARAP GABA(A) receptor-associated protein [ Homo sapiens ]
• Official Symbol: GABARAP
• Synonyms: GABARAP; GABA(A) receptor-associated protein; gamma-aminobutyric acid receptor-associated protein; ATG8A; MM46; GABARAP-a; FLJ25768; MGC120154; MGC120155
• Gene ID: 11337
• mRNA Refseq: NM_007278
• Protein Refseq: NP_009209
• MIM: 605125
• UniProt ID: O95166
• Chromosome Location: 17p13.1