Active Recombinant Human G Protein-Coupled Receptor Associated Sorting Protein 2, His-tagged

• Cat.No.: GPRASP2-5634H
• Product Overview: RecombinantHuman GPRASP2 protein was expressed in Murine myeloma cellline. Ala20-Asp548,with a C-terminal 6-His tag.

Specification

• Cat. No.: GPRASP2-5634H
• Description: GPRASP2cDNA encodes a 548 amino acid protein that contains a 19 amino acid signalsequence and is comprised of many conserved domains: WAP, follistatin/Kazal,immunoglobulin, two tandem Kunitz, and netrin. Based on the order of theseconserved domains, GPRASP2 is also known as WFIKKN. Another related proteinwhich contains the same domain structure is called WFIKKNRP (WFIKKN-relatedprotein), or GASP-1. WAP, follistatin, Kunitz and netrin domains are allimplicated in protease inhibition, and the GASP proteins may be multivalent proteaseinhibitors.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 um filtered solution in PBS with BSA as a carrier protein.
• N-terminalSequence: Ala20
• MolecularWeight: 57.5 kDa
• Activity: Measuredby its ability to inhibit rmGDF-8 activity in K562 human chronic myelogenousleukemia cells. The ED50 for this effect is typically 0.25-1.25 μg/mL, in thepresence of 40 ng/mL of rmGDF-8.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 65-72kDa, reducing conditions.
• Purity: >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mLin sterile PBS containing at least 0.1% human or bovine serum albumin
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 monthsfrom date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C understerile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution.
• Protein length: 20-548 a.a.

Gene Information

• Gene Name: GPRASP2 G protein-coupledreceptor associated sorting protein 2 [ Homo sapiens ]
• Official Symbol: GPRASP2
• Synonyms: GPRASP2; Gprotein-coupled receptor associated sorting protein 2; GASP2;G-protein coupled receptor-associated sorting protein 2; FLJ37327;GASP-2
• Gene ID: 114928
• mRNA Refseq: NM_001184876
• Protein Refseq: NP_001171805
• UniProt ID: Q96D09
• Chromosome Location: Xq22.1