Active Recombinant Human G Protein-Coupled Receptor 115, His-tagged

• Cat.No.: GPR115-5631H
• Product Overview: RecombinantHuman GPR115 protein was expressed in Chinese HamsterOvary cell line. Ser22-Ala347, with a C-terminal 6-Histag.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 22-347 a.a.
• Description: GPR115 is a member of the LN-7TM family of adhesion-type7-transmembrane (TM) G-protein coupled receptors (GPCR) that show a longextracellular N-terminus. The 695 amino acid (aa) human GPR115 sequencepredicts a 21 aa signal sequence, a 385 aa N-terminal extracellular domain(ECD), seven TM regions separated by 6-24 aa intracellular and extracellularregions, and a 40 aa cytoplasmic tail. Like other LN-7TM members, the ECDcontains a highly glycosylated mucin-like stalk that is predicted to functionin adhesion. This is followed by a cysteine-rich GPCR proteolytic cleavagesite (GPS).
• Form: Lyophilized from a0.2 um filtered solution in PBS.
• N-terminalSequence: Ser22
• MolecularWeight: 37 kDa
• Activity: Measuredby the ability of the immobilized protein to support the adhesion of U-87 MGhuman glioblastoma/astrocytoma cells. When 5x104 cells per wellare added to rhGPR115 coated plates (5 μg/mL, 100 μL/well), approximately 60-85%will adhere after 60 minutes at 37°C.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 66-100kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 200 μg/mLin sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 monthsfrom date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C understerile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution.

Gene Information

• Gene Name: GPR115 G protein-coupledreceptor 115 [ Homo sapiens ]
• Official Symbol: GPR115
• Synonyms: GPR115; Gprotein-coupled receptor 115; PGR18; probable G-protein coupled receptor115; G-protein coupled receptor PGR18; seven transmembrane helix receptor
• Gene ID: 221393
• mRNA Refseq: NM_153838
• Protein Refseq: NP_722580
• MIM: 614268
• UniProt ID: Q8IZF3
• Chromosome Location: 6p12.3
• Function: G-protein coupledreceptor activity; receptor activity; signal transducer activity