Active Recombinant Human G Protein-Coupled Receptor 114, His-tagged

• Cat.No.: GPR114-5630H
• Product Overview: RecombinantHuman GPR114 protein was expressed in Chinese HamsterOvary cell line. Glu22-Gly184, with a C-terminal 6-His tag.

Specification

• Species: Human
• Source: CHO
• Tag: His
• ProteinLength: 22-184 a.a.
• Description: GPR114 isa member of the LN-TM7 family of adhesion-type 7-transmembrane (TM) G-proteincoupled receptors (GPCR) that exhibit long extracellular N-termini. The 528amino acid (aa) human GPR114 sequence predicts a 21 aa signal sequence, a 229aa N-terminal extracellular domain (ECD) and seven TM regions separated by shortintracellular and extracellular regions. Like other LN-TM7 members, the ECDcontains a highly glycosylated mucin-like stalk that is predicted to functionin adhesion, followed by a GPCR proteolytic cleavage site (GPS) it does not containany other easily recognizable domains.
• Form: Lyophilized from a0.2 um filtered solution in PBS.
• N-terminalSequence: Glu22
• MolecularWeight: 19.4 kDa
• Activity: Measuredby the ability of the immobilized protein to enhance the adhesion of thehuman T98G glioblastoma cells (ATCC: CRL-1690) to human Fibronectin. When 4x104cells/well are added to plates coated with human Fibronectin (0.1 μg/mL) andrhGPR114 (10 μg/well, 100 μL/well) for 60 minutes at 37°C, there is a 2-4fold increase in adhesion to human fibronectin induced by the presence ofrhGPR114.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 40-55kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100μg/mL in PBS.
• Storage: Use a manual defrost freezer and avoid repeated freezethawcycles. 12months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °Cunder sterile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution.

Gene Information

• Gene Name: GPR114 G protein-coupledreceptor 114 [ Homo sapiens ]
• Official Symbol: GPR114
• Synonyms: GPR114; Gprotein-coupled receptor 114; PGR27; probable G-proteincoupled receptor 114; G protein-coupled receptor PGR27; G-protein coupledreceptor PGR27
• Gene ID: 221188
• mRNA Refseq: NM_153837
• Protein Refseq: NP_722579
• UniProt ID: Q8IZF4
• Chromosome Location: 16q13
• Function: G-protein coupledreceptor activity; receptor activity; signal transducer activity