Active Recombinant Human Fucosyltransferase 3 (galactoside 3 (4)-L-fucosyltransferase, Lewis blood group), His-tagged

• Cat.No.: FUT3-573H
• Product Overview: Recombinanthuman FUT3 protein was expressed in Murine myeloma cell line, NS0-derived,with an N-terminus 6-His tag. Arg35-Thr361.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 35-361 a.a.
• Description: FUT3 is often foundto be essential for sugar epitope and lectin ligand generation. Well knownFUT3 containing structures include Lewis structures and ABO blood groupantigens. Lewis structures are key elements involved in leukocyte homing and extravasationprocess and thus are essential for lymphocyte maturation and natural defense functions.
• Form: Supplied as a 0.2 μmfiltered solution in Tris and NaCl.
• N-terminal Sequence: His
• Molecular Weight: 39 kDa
• Activity: Measured by itsability to transfer fucose from GDP-fucose to N-Acetyllactosamine. Thespecific activity is > 25 pmol/min/μg, as measured under the described conditions.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• SDS-PAGE: 41 kDa, reducingconditions
• Purity: >90%, by SDS-PAGEunder reducing conditions and visualized by silver stain.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3months, -20 to -70 °C under sterile conditions after reconstitution.

Gene Information

• Gene Name: FUT3 fucosyltransferase 3(galactoside 3(4)-L-fucosyltransferase, Lewis blood group) [ Homo sapiens]
• Official Symbol: FUT3
• Synonyms: FUT3;fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis bloodgroup); fucosyltransferase 3 (galactoside 3(4) L fucosyltransferase, Lewisblood group included); LE; galactoside 3(4)-L-fucosyltransferase; CD174;MGC131739; alpha (1,3/1,4) fucosyltransferase; Blood group Lewisalpha-4-fucosyltransferase; FT3B; Fucosyltransferase 3; FucosyltransferaseIII; FucT-III; FUT3_HUMAN; galactoside 3(4) L fucosyltransferase, Lewis bloodgroup; Galactoside 3(4)-L-fucosyltransferase; Lewis B Blood Group antigen;Lewis FT; alpha-(1,3/1,4)-fucosyltransferase; Les
• Gene ID: 2525
• mRNA Refseq: NM_000149
• Protein Refseq: NP_000140
• MIM: 111100
• UniProt ID: P21217
• Chromosome Location: 19p13.3
• Pathway: Glycosphingolipidbiosynthesis-lacto and neolacto series; Metabolic pathways
• Function: 3-galactosyl-N-acetylglucosaminide4-alpha-L-fucosyltransferase activity; alpha-(1->3)-fucosyltransferaseactivity; fucosyltransferase activity; transferase activity, transferringglycosyl groups