Active Recombinant Human Fucosidase, Alpha-L- 1, Tissue, His-tagged

• Cat.No.: FUCA1-471H
• Product Overview: Recombinant Human FUCA1protein was expressed in Murine myeloma cell line with an N-terminal CD33 signalsequence and a C-terminal 6-His tag. Gln32-Lys466.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 32-466 a.a.
• Description: Fucosylated glycoconjugatesplay numerous roles in biological processes, such as development, apoptosis andthe immune responses, and are involved in the pathology of inflammation, cancerand cystic fibrosis. Tissue α LFucosidase, also known as FUCA1, is a lysosomalenzyme responsible for hydrolyzing αLfucose moieties from glycolipids and oligosaccharides.
• Form: Supplied as a 0.2 μmfiltered solution in Tris and NaCl.
• N-terminalSequence: Gln32
• Molecular Weight: 51 kDa
• Activity: Measuredby its ability to cleave a fluorogenic substrate 4-methylumbelliferyl-α-L-fucopyranoside.The specific activity is > 4800 pmol/min/μg, as measured under the describedconditions. See Activity Assay Protocol.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Bluestain at 5 μg per lane.
• Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 6 months from date of receipt,20 to 70 °C as supplied. 3 months, 20 to 70°C under sterile conditions after opening.

Gene Information

• Gene Name: FUCA1 fucosidase, alpha-L- 1, tissue[ Homo sapiens ]
• Official Symbol: FUCA1
• Synonyms: FUCA1; fucosidase,alpha-L- 1, tissue; FUCA; tissue alpha-L-fucosidase; alpha-L-fucosidase 1; alpha-L-fucosidaseI; alpha-L-fucoside fucohydrolase 1; EC 3.2.1.51; Nbla10230
• Gene ID: 2517
• mRNA Refseq: NM_000147
• Protein Refseq: NP_000138
• MIM: 612280
• UniProt ID: P04066
• Chromosome Location: 1p34
• Pathway: Lysosome; Other glycandegradation
• Function: alpha-L-fucosidase activity;cation binding; hydrolase activity; sugar bindin