Active Recombinant Human Frizzled Family Receptor 8, Fc-tagged

• Cat.No.: FZD8-597H
• Product Overview: RecombinantHuman FZD8 protein was expressed in Chinese Hamster Ovary cell line with theFc part of Human IgG1 Pro100-Lys330 (C-terminus) and Human Frizzled-8 Ala25-Pro172(N-terminus).

Specification

• Cat. No.: FZD8-597H
• Description: FZD8 is one of atleast ten seventransmembrane (7TM) glycoprotein of the Frizzled family of Wntreceptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement,with low density lipoprotein receptor related proteins LRP-5 or LRP-6 actingas co-receptors stabilizes β-catenin and promotes gene transcription that isimportant in development and tissue maintenance.
• Source: Chinese HamsterOvary cell line, CHO-derived
• Form: Supplied as a 0.2 μmfiltered solution in PBS.
• N-terminal Sequence: Ala25 & Ala28
• Molecular Weight: 43.3 kDa
• Activity: Measured by itsbinding ability in a functional ELISA. In a 100 μL reaction mixturecontaining biotinylated rmWnt-3a or rmWnt-5a at 50-100 ng/mL and rhFrizzled8/Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled8/ FcChimera that produces 50% of the optimal binding response is found to beapproximately 7-28 ng/mL.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• SDS-PAGE: 55-65 kDa, reducingconditions
• Purity: >95%, by SDS-PAGEunder reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 500μg/mL in sterile PBS.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to-70 °C under sterile conditions after reconstitution.
• Protein length: 25-172 a.a.

Gene Information

• Gene Name: FZD8 frizzled family receptor 8[ Homo sapiens ]
• Official Symbol: FZD8
• Synonyms: FZD8; frizzledfamily receptor 8; FZ-8; hFZ8; frizzled (Drosophila) homolog 8;frizzled 8, seven transmembrane spanning receptor; frizzled homolog 8(Drosophila); OTTHUMP00000019454; hFz8; Fz-8; frizzled-8; frizzledhomolog 8
• Gene ID: 8325
• mRNA Refseq: NM_031866
• Protein Refseq: NP_114072
• MIM: 606146
• UniProt ID: Q9H461
• Chromosome Location: 10p11.2
• Pathway: Basal cellcarcinoma; Class B/2 (Secretin family receptors); GPCR ligand binding; HTLV-Iinfection; Melanogenesis; Pathways in cancer; Signal Transduction; Signalingby GPCR; Wnt Signaling Pathway; Wnt signaling network
• Function: G-proteincoupled receptor activity; PDZ domain binding; Wnt-activated receptoractivity; Wnt-protein binding