Active Recombinant Human Follistatin-Like 4, His-tagged

• Cat.No.: FSTL4-563H
• Product Overview: Recombinanthuman FSTL4 protein was expressed in Chinese Hamster Ovary cell line, CHO-derived,with an N-terminus 7-His tag and Asn. Trp23-Val842.

Specification

• Cat. No.: FSTL4-563H
• Description: FSTL4 is apresumably secreted member of the follistatin domain-containing protein family.FSTL4 itself is a potent inhibitor of the TGF-β superfamily member, activin,and this activity is reflected in several other proteins of this family thatcontain varying numbers of the cysteine-rich follistatin domain.
• Source: Chinese HamsterOvary cell line, CHO-derived
• Form: Lyophilizedfrom a 0.2 μm filtered solution in PBS.
• N-terminal Sequence: His
• Molecular Weight: 93.6 kDa
• Activity: Measured by itsability to inhibit BMP-6-induced alkaline phosphatase production by MC3T3-E1 mouseosteoblastic cells. The ED50 for this effect is typically 2-10 μg/mLin the presence of 0.1 μg/mL of recombinant human BMP-6.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• SDS-PAGE: 100 kDa, reducingconditions
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 300μg/mL in sterile PBS.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month,2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions afterreconstitution.
• Protein length: 23-842 a.a.

Gene Information

• Gene Name: FSTL4 follistatin-like 4 [ Homosapiens ]
• Official Symbol: FSTL4
• Synonyms: FSTL4; follistatin-like 4; follistatin-related protein 4;KIAA1061; Follistatin like 4; Follistatin related protein 4; FSTL 4;follistatin-like protein 4; OTTHUMP00000222585; OTTHUMP00000222586
• Gene ID: 23105
• mRNA Refseq: NM_015082
• Protein Refseq: NP_055897
• UniProt ID: Q6MZW2
• Chromosome Location: 5q31.1
• Function: calcium ion binding