Active Recombinant Human FLT4 protein, His-tagged

• Cat.No.: FLT4-0239H
• Product Overview: Recombinant Human FLT4 protein(P17948-1)(Met1-Asn756), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC) (Conn, G. et al.,1990 , Proc. Natl. Acad. Sci. USA 87:1323.). The ED50 for this effect is typically 10-40 ng/mL in the presence of 10 ng/mL human VEGF165.
• Molecular Mass: The secreted recombinant human VEGFR1 consists of 741 amino acids with the predicted molecular mass of 83.7 kDa. As a result of glycosylation, rhVEGFR1 migrates as an approximately 110-120 kDa band in SDS-PAGE under reducing conditions.
• Protein length: Met1-Asn756
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FLT4 fms-related tyrosine kinase 4 [ Homo sapiens ]
• Official Symbol: FLT4
• Synonyms: FLT4; fms-related tyrosine kinase 4; vascular endothelial growth factor receptor 3; PCL; VEGFR3; FLT-4; VEGFR-3; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; fms-like tyrosine kinase 4; tyrosine-protein kinase receptor FLT4; FLT41; LMPH1A
• Gene ID: 2324
• mRNA Refseq: NM_002020
• Protein Refseq: NP_002011
• MIM: 136352
• UniProt ID: P35916

Reviews

09/05/2022

The catalytic activity of FLT4 is very strong, which can significantly improve the selectivity and yield of chemical reactions, and its application potential is great.

09/26/2019

We were reassured that the quality of FLT4 was consistent.

03/23/2019

Using FLT4 for experiments, I can focus more on experimental design and data analysis without worrying about the reproducibility of experimental results.

Q&As

Does FLT4 protein correlate with tumor-related markers?

FLT4 protein is associated with various tumor-related markers such as malignant lymphoma, breast cancer, and lung cancer.

Is there a relationship between FLT4 protein and lymphedema?

FLT4 protein is a key regulator of lymphedema and plays an important role in lymphangiogenesis and lymphatic drainage.

Is FLT4 protein associated with congenital lymphedema?

Yes, mutations in the FLT4 protein can lead to congenital lymphedema, a condition caused by abnormal lymphatic vessel development.

What is the potential of FLT4 protein in cancer treatment?

The protein is considered an important target in anticancer therapy, and inhibition of its activity may reduce tumor angiogenesis and metastasis.

What is the role of FLT4 protein in pulmonary hypertension?

FLT4 protein plays an important role in pulmonary hypertension, and upregulation of its activity may lead to pathological changes such as vascular remodeling and pulmonary artery stenosis.

What is the role of FLT4 protein in angiogenesis?

This is involved in the regulation of angiogenesis and lymphangiogenesis by binding to vascular endothelial growth factor C (VEGF-C) and vascular endothelial growth factor D (VEGF-D).