Active Recombinant Human FLT3LG Protein (Thr27-Ala181), GMP Grade, Animal-Free

• Cat.No.: FLT3LG-32HG
• Product Overview: GMP recombinant human Flt-3 Ligand/FLT3L protein (Thr27-Ala181) without tag was expressed in E. coli, produced in an Animal-Free laboratory and manufactured and tested under cGMP guidelines.

Specification

• Species: Human
• Source: E.coli
• Protein Length: Thr27-Ala181
• Description: Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts.
• Bio-activity: Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with mouse Flt-3. The ED50 for this effect is 0.2-1 ng/mL.
  The specific activity of Recombinant Human Flt-3 Ligand/FLT3L is >5.0 x 105 units/mg, which is calibrated against the human Flt-3 Ligand WHO Standard.
• Molecular Mass: Predicted Molecular Mass: 18 kDa
  SDS-PAGE: 15-17 kDa, under reducing conditions
• N-terminal Sequence Analysis: Met-Thr27-Gln-Asp-(Cys)-Ser-Phe-Gln-His-Ser and Thr27-Gln-Asp-(Cys)-Ser-Phe-Gln-His-Ser-Pro
• Endotoxin: <0.10 EU/ μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  A minimum of 6 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100-500 μg/mL in PBS.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: FLT3LG fms-related tyrosine kinase 3 ligand [ Homo sapiens ]
• Official Symbol: FLT3LG
• Synonyms: FLT3LG; fms-related tyrosine kinase 3 ligand; flt3 ligand; FL; FLT3L
• Gene ID: 2323
• mRNA Refseq: NM_001204502
• Protein Refseq: NP_001191431
• MIM: 600007
• UniProt ID: P49771