Active Recombinant Human FLT3L, His-tagged

• Cat.No.: FLT3L-20H
• Product Overview: Recombinant Human FLT3L was purified from the endosperm tissue of barley grain (Hordeum vulgare).

Specification

• Species: Human
• Source: Barley Grain
• Tag: His
• Description: Flt3-ligand (FMS related tyrosine kinase 3 ligand, flk-2 ligand) is involved in proliferation and differentiation of early hematopietic cells. Flt3-ligand synergizes with other CSF"s and interleukins to induce proliferation of early hematopoietic cells, but does not stimulate growth or differentation alone. Flt3-ligand binds to cells expressing the tyrosine kinase receptor Flt3. Multiple isoforms of Flt3-ligand have been identified but the predominant biologically active isoform is a transmembrane protein which can be proteolytically cleaved to generate a biologically active soluble form.
• Form: PBS pH 7.2, sterile filtered
• Bio-activity: Recombinant human Flt-3 ligand was determined by its dose-dependent effects on the proliferation of AML5 cells. The ED50 value is typically< 0.5="" ng/ml="" corresponding="" to="" specific="" activity="">2 x 10e6 U/mg. Optimal concentration should be determined for specific applications and cell lines.
• Molecular Mass: Recombinant human Flt3-ligand contains 156 amino acids and a 16 a.a. Histidine-based tag for a total length of 172 a.a. and has a predicted molecular mass of 19.9 kDa. As a result of glycosylation, the recombinant protein migrates as two bands with an apparent molecular mass of 20 and 22 kDa in SDS-PAGE.
• Endotoxin: Endotoxin level is less than 0.005ng per μg of product (0.05EU/μg).
• Purity: Greater than 95% by SDS-PAGE gel analysis.
• Storage: The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
• Reconstitution: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 μg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA).