Active Recombinant Human Flt3 protein, His-Avi-tagged, Biotinylated

• Cat.No.: Flt3-1430H
• Product Overview: Biotinylated Recombinant Human Flt3 protein(Thr27-Pro185), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His&Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Immobilized Human Flt-3, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human Flt-3 Ligand, His,Avitag with a linear range of 0.1-4 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 21.6 kDa. The protein migrates as 26-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Thr27-Pro185
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: Thr27-Pro185

Gene Information

• Gene Name: FLT3 fms-related tyrosine kinase 3 [ Homo sapiens ]
• Official Symbol: Flt3
• Synonyms: FLT3; fms-related tyrosine kinase 3; receptor-type tyrosine-protein kinase FLT3; CD135; FLK2; STK1; STK-1; CD135 antigen; FL cytokine receptor; fetal liver kinase 2; fms-like tyrosine kinase 3; stem cell tyrosine kinase 1; growth factor receptor tyrosine kinase type III; FLK-2
• Gene ID: 2322
• mRNA Refseq: NM_004119
• Protein Refseq: NP_004110
• MIM: 136351
• UniProt ID: P36888

Reviews

08/01/2021

Excellent product, great price.

12/22/2020

Satisfied, will order again.

09/14/2020

Quick response, helpful service.

Q&As

How is FLT3 implicated in acute myeloid leukemia (AML)?

FLT3 is frequently mutated in AML, contributing to uncontrolled cell growth and poor prognosis.

In which cells or tissues is FLT3 predominantly expressed?

FLT3 is predominantly expressed in hematopoietic stem cells and progenitor cells.

How does FLT3 contribute to hematopoiesis?

It is essential for hematopoiesis, particularly in the proliferation of early progenitor cells.

Are there therapeutic strategies targeting FLT3 for disease treatment?

Therapeutic strategies include FLT3 inhibitors, showing effectiveness in treating AML patients with FLT3 mutations.

What is the primary biological function of FLT3 in cells?

FLT3 is a receptor tyrosine kinase that plays a crucial role in cell survival, proliferation, and differentiation.

What role does FLT3 play in the proliferation and differentiation of hematopoietic stem cells?

It stimulates the proliferation and differentiation of hematopoietic stem cells, guiding their development into various blood cell types.

What are the common mutations found in FLT3 associated with diseases?

Common mutations include internal tandem duplications (ITD) and tyrosine kinase domain (TKD) mutations, leading to constitutive activation of FLT3.