Active Recombinant Human Fibroblast Growth Factor 3

• Cat.No.: FGF3-518H
• Product Overview: RecombinantHuman FGF3 protein was expressed in E. coli. Asp28-Arg212,with an N-terminal Met.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 28-212 a.a.
• Description: FGF3 belongs to the large FGF family which has at least 23members. All FGF family members are heparin-binding growth factors with acore 120 amino acid (aa) FGF domain that allows for a common tertiarystructure. FGFs are expressed during embryonic development and in restrictedadult tissues. They act on cells of mesodermal and neuroectodermal origin toregulate diverse physiologic functions including angiogenesis, cell growth,pattern formation, embryonic development, metabolic regulation, cellmigration, neurotrophic effects and tissue repair. Signaling receptors forFGFs are type I transmembrane receptor tyrosine kinases belonging to the Igsuperfamily.
• Form: Lyophilizedfrom a 0.2 um filtered solution in MOPS, Na2SO4 and EDTA with BSA as acarrier protein.
• N-terminalSequence: Met
• MolecularWeight: 21.1 kDa
• Activity: Measuredin a cell proliferation assay using NR6R3T3 mouse fibroblasts. The ED50 forthis effect is typically 0.02 0.1 μg/mL in the presence of 1 μg/mL of heparin.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• Purity: >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 10μg/mL in sterile PBS containing at least 0.1% human or bovine serumalbumin.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: FGF3 fibroblast growth factor 3[ Homo sapiens ]
• Official Symbol: FGF3
• Synonyms: FGF3; fibroblastgrowth factor 3; fibroblast growth factor 3 (murine mammary tumor virusintegration site (v int 2) oncogene homolog); INT2; HBGF 3; INT 2 protooncogene protein; oncogene INT2; VINT2 murine mammary tumor virus integrationsite oncogene homolog; INT2 proto oncogene protein precurosr; FGF-3;OTTHUMP00000237184; proto-oncogene Int-2; INT-2 proto-oncogene protein;heparin-binding growth factor 3; murine mammary tumor virus integration site2, mouse; V-INT2 murine mammary tumor virus integration site onc
• Gene ID: 2248
• mRNA Refseq: NM_005247
• Protein Refseq: NP_005238
• MIM: 164950
• UniProt ID: P11487
• Chromosome Location: 11q13
• Pathway: Downstream signaling of activated FGFR; FGFRligand binding and activation; FGFR1 ligand binding and activation; FRS2-mediatedcascade; IRS-mediated signalling; IRS-related events; Insulin receptorsignalling cascade; MAPK signaling pathway; Melanoma; Negative regulation ofFGFR signaling; PI-3K casrcade; Pathwaysin cancer; Phospholipase C-mediated cascade; Regulation of Actin Cytoskeleton;SHC-mediated cascade; Signaling by FGFR
• Function: growth factor activity; proteinbinding