Active Recombinant Human FGF2 Protein, Biotinylated

• Cat.No.: FGF2-13H
• Product Overview: Biotinylated Human FGF basic is expressed from E.coli. It contains AA Pro 143 - Ser 288 (Accession # NP_001997). The product does not contain any epitope tags.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: Pro143-Ser288
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1.iPSC-derived human vascular organoids (Day 7) were cultured with FGF2, VEGFA, EGF. Red arrows represent vascular organoids. Image taken at 10x magnification.(Routinely tested)
  2.Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts.
  The ED50 for this effect is typically 0.02-0.1 ng/ml.
• Molecular Mass: The recombinant human bFGF consists of 147 amino acids and predicts a molecular mass of 16.5 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: FGF2 fibroblast growth factor 2 (basic) [ Homo sapiens ]
• Official Symbol: FGF2
• Synonyms: FGF2; fibroblast growth factor 2 (basic); FGFB; fibroblast growth factor 2; prostatropin; heparin-binding growth factor 2; basic fibroblast growth factor bFGF; BFGF; FGF-2; HBGF-2
• Gene ID: 2247
• mRNA Refseq: NM_002006
• Protein Refseq: NP_001997
• MIM: 134920
• UniProt ID: P09038