Active Recombinant Rat Fgf2 Protein (146 aa)

• Cat.No.: Fgf2-397F
• Product Overview: Recombinant rat Fibroblast Growth Factor-basic (rrFGF-basic) produced in E. coli is a single non-glycosylated polypeptide chain containing 146 amino acids. A fully biologically active molecule, rrFGF-basic has a molecular mass of 16.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.

Specification

• Species: Rat
• Source: E.coli
• Protein Length: 146
• Description: Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the β trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: ED50 < 0.25 ng/mL, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 4 × 10^6 units/mg.
• Molecular Mass: 16.4 kDa, observed by reducing SDS-PAGE.
• AA Sequence: GPALPEDGGGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHVKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTEECFFFERLESNNYNTYRSRKYSSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% by SDS-PAGE analysis.
• Storage: Lyophilized recombinant rat Fibroblast Growth Factor-basic (rrFGF-basic) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rrFGF-basic remains stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Gene Information

• Gene Name: Fgf2 fibroblast growth factor 2 [ Rattus norvegicus ]
• Official Symbol: Fgf2
• Synonyms: FGF2; fibroblast growth factor 2; HBGF-2; basic fibroblast growth factor; heparin-binding growth factor 2; bFGF; Fgf-2
• Gene ID: 54250
• mRNA Refseq: NM_019305
• Protein Refseq: NP_062178
• UniProt ID: P13109