Active Recombinant Human FGF2 (143-288aa), Ala tagged

• Cat.No.: FGF2-022E
• Product Overview: Recombinant Bovine FGF2 (Pro143 ­ Ser288, with an N­terminal Ala) was expressed in E. coli and produced using non­animal reagents in an animal­free laboratory.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: FGF basic stimulates the proliferation of all cells of mesodermal origin, and many cells of neuroectodermal, ectodermal and endodermal origin. The cells include fibroblasts, endothelial cells, astrocytes, oligodendrocytes, neuroblasts, keratinocytes, osteoblasts, smooth muscle cells, and melanocytes. FGF basic is chemotactic and mitogenic for endothelial cells in vitro. FGF basic induces neuron differentiation, survival and regeneration. FGF basic has also been shown to be crucial in modulating embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.
• N­terminal Sequence Analysis: Ala
• Molecular Mass: 16.0 kDa
• SDS­PAGE: 17 kDa under reducing conditions
• Activity: Measured in a cell proliferation assay using NR6R­3T3 mouse fibroblasts. The ED50 for this effect is typically 0.1 ­ 0.6 ng/mL.
• Endotoxin Leve: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
• Formulation: Lyophilized from a 0.2 μm filtered solution in Tris­HCl and NaCl. See Certificate of Analysis for details.
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3. 3 months, ­20 to ­70 °C under sterile conditions after reconstitution.
• Unitprot ID: P09038

Gene Information

• Gene Name: FGF2 fibroblast growth factor 2 (basic) [ Homo sapiens ]
• Official Symbol: FGF2
• Synonyms: FGF2; fibroblast growth factor 2 (basic); BFGF; FGFB; HBGF-2; heparin-binding growth factor 2; prostatropin; basic fibroblast growth factor bFGF
• Gene ID: 2247
• mRNA Refseq: NM_002006
• Protein Refseq: NP_001997
• MIM: 134920
• Chromosome Location: 4q26
• Pathway: Angiopoietin receptor Tie2-mediated signaling; Downstream signaling of activated FGFR; Endochondral Ossification; FGFR ligand binding and activation; FGFR1 ligand binding and activation; FGFR2 ligand binding and activation; FGFR3 ligand binding and activation; FGFR4 ligand binding and activation; IRS-mediated signaling; MAPK signaling pathway; Regulation of Actin Cytoskeleton
• Function: fibroblast growth factor binding; fibroblast growth factor receptor binding; heparin binding; protein binding; protein tyrosine kinase activity; voltage-gated calcium channel activity