Active Recombinant Human FGF1 (16-155aa), Met tagged

• Cat.No.: FGF1-021E
• Product Overview: Recombinant Human FGF1 (Phe16 ­ Asp155, with an N­terminal Met) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: FGF acidic, also known as FGF­1, ECGF, and HBGF­1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis.
• N­terminal Sequence Analysis: Met
• Molecular Mass: 15.5 kDa
• Activity: Measured in a cell proliferation assay using NR6R­3T3 mouse fibroblasts. The ED50 for this effect is typically 0.1 ­ 0.3 ng/mL in the presence of 10 µg/mL of heparin.
• Endotoxin Leve: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
• Formulation: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and DTT. See Certificate of Analysis for details.
• Reconstitution: Reconstitute at 200 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3. 3 months, ­20 to ­70 °C under sterile conditions after reconstitution.
• Unitprot ID: P05230

Gene Information

• Gene Name: FGF1 fibroblast growth factor 1 (acidic) [ Homo sapiens ]
• Official Symbol: FGF1
• Synonyms: FGF1; fibroblast growth factor 1 (acidic); AFGF; ECGF; FGFA; ECGFA; ECGFB; HBGF1; GLIO703; ECGF-beta; FGF-alpha; heparin-binding growth factor 1; HBGF-1; acidic fibroblast growth factor; beta-endothelial cell growth factor; endothelial cell growth factor, beta; endothelial cell growth factor, alpha
• Gene ID: 2246
• mRNA Refseq: NM_000800
• Protein Refseq: NP_000791
• MIM: 131220
• Chromosome Location: 5q31
• Pathway: Downstream signaling of activated FGFR; FGF signaling pathway; FGFR ligand binding and activation; FRS2-mediated cascade; IRS-mediated signaling; Insulin receptor signalling cascade; MAPK signaling pathway; PI3K Cascade; Regulation of actin cytoskeleton
• Function: S100 alpha binding; fibroblast growth factor receptor binding; growth factor activity; heparin binding; protein tyrosine kinase activity; protein binding