Active Recombinant Human FCGRT & B2M Heterodimer Protein, His & Avi & Strep II-tagged, Biotinylated

• Cat.No.: FCGRT & B2M-377H
• Product Overview: Recombinant Human FCGRT & B2M Heterodimer Protein, His & Avi & Strep II-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P55899-1 (FCGRT) & P61769-1 (B2M)). Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M). Biotinylated Human FcRn Heterodimer Protein (SPR verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.1 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 38 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His&Strep II
• Protein Length: Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Recombinant Human FCGRT & B2M Heterodimer Protein, His & Avi & Strep II-tagged, Biotinylated captured on Biotin CAP- Series S Sensor Chip can bind Herceptin with an affinity constant of 0.369 μM as determined in a SPR assay (Biacore T200) (QC tested).
• Molecular Mass: 34.1 kDa (FCGRT) & 13.1 kDa (B2M)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: FCGRT Fc fragment of IgG, receptor, transporter, alpha [ Homo sapiens ] & B2M beta-2-microglobulin [ Homo sapiens ]
• Official Symbol: FCGRT & B2M
• Synonyms: FCGRT; alpha chain; FCRN; FcRn alpha chain; alpha-chain; & B2M; beta-2-microglobulin
• Gene ID: 2217 & 567
• mRNA Refseq: NM_001136019 & NM_004048
• Protein Refseq: NP_001129491 & NP_004039
• MIM: 601437 & 109700
• UniProt ID: P55899 & P61769