Active Recombinant Human FCGR2A Protein, His-tagged, Biotinylated

• Cat.No.: FCGR2A-260H
• Product Overview: Biotinylated Human Fc gamma RIIA / CD32a (H131) (CDA-H82E6) is expressed from human HEK293 cells. It contains AA Ala 36 - Ile 218 (Accession # P12318-1).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 36-218 a.a.
• Predicted N Terminal: Ala 36
• Form: Lyophilized from sterile PBS, pH7.4, 10% Trehalose.
• Bio-activity: The bioactivity is measured by its binding ability to Ipilimumab in a SPR assay. Immobilized Human FcGR2A / CD32a (H131), His Tag can bind Ipilimumab with affinity constant of around 2.3 uM. Measured by its binding ability in a functional ELISA against immobilized Yervoy Ipilimumab (0.5 ug/well). The linear range is 0.5-16 ng/mL.
• Molecular Mass: This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 24 kDa. The protein migrates as 30-38 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: FCGR2A Fc fragment of IgG, low affinity IIa, receptor (CD32) [ Homo sapiens ]
• Official Symbol: FCGR2A
• Synonyms: FCGR2A; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32) , FCG2, FCGR2, FCGR2A1; low affinity immunoglobulin gamma Fc region receptor II-a; CD32; CD32A; CDw32; IGFR2; Immunoglobulin G Fc receptor II; fcRII-a; fc-gamma-RIIa; fc-gamma RII-a; igG Fc receptor II-a; FCG2; FcGR; FCGR2; FCGR2A1; MGC23887; MGC30032
• Gene ID: 2212
• mRNA Refseq: NM_001136219
• Protein Refseq: NP_001129691
• MIM: 146790
• UniProt ID: P12318
• Chromosome Location: 1q23
• Pathway: Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Leishmaniasis, organism-specific biosystem; Leishmaniasis, conserved biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem; Phagosome, organism-specific biosystem
• Function: IgG binding; receptor activity