Active Recombinant Human FCGR2A protein(167 Arg), His-tagged

Cat.No. : FCGR2A-649H
Product Overview : Recombinant Human FCGR2A protein (Met1-Ile218) (P12318-1, with Natural variant His 167 Arg), fused with a C-terminal polyhistidine tag, was expressed in HEK293.
Availability March 12, 2025
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Species : Human
Source : HEK293
Tag : His
Protein Length : Met1-Ile218
Form : Lyophilized from sterile PBS, pH 7.5
Normally 5 % trehalose, 5 % mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : 1. Measured by its ability to bind biotinylated human IgG2 in functional ELISA.
2. Loaded Anti-CD32A Antibody, Mouse Monoclonal on AMC Biosensor, can bind CD32A Protein, Human, Recombinant (167 Arg, His Tag) with an affinity constant of 6.8 nM as determined by Octet RED System.
Molecular Mass : The recombinant human FCGR2A consists of 196 amino acids after removal of the signal peptide and predicts a molecular mass of 22 kDa. It migrates as an approximately 30 kDa protein due to glycosylation by SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 97 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name FCGR2A Fc fragment of IgG, low affinity IIa, receptor (CD32) [ Homo sapiens ]
Official Symbol FCGR2A
Synonyms FCGR2A; CD32; CD32A; CDw32; IGFR2; fcRII-a; fc-gamma-RIIa; FCG2; FcGR; FCGR2; FCGR2A1; MGC23887; MGC30032;
Gene ID 2212
mRNA Refseq NM_001136219.1
Protein Refseq NP_001129691.1
MIM 146790
UniProt ID P12318

Not For Human Consumption!

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