Active Recombinant Human FCGR2A protein(167 Arg), His-tagged
Cat.No. : | FCGR2A-649H |
Product Overview : | Recombinant Human FCGR2A protein (Met1-Ile218) (P12318-1, with Natural variant His 167 Arg), fused with a C-terminal polyhistidine tag, was expressed in HEK293. |
Availability | March 12, 2025 |
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Species : | Human |
Source : | HEK293 |
Tag : | His |
Protein Length : | Met1-Ile218 |
Form : | Lyophilized from sterile PBS, pH 7.5 Normally 5 % trehalose, 5 % mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured by its ability to bind biotinylated human IgG2 in functional ELISA. 2. Loaded Anti-CD32A Antibody, Mouse Monoclonal on AMC Biosensor, can bind CD32A Protein, Human, Recombinant (167 Arg, His Tag) with an affinity constant of 6.8 nM as determined by Octet RED System. |
Molecular Mass : | The recombinant human FCGR2A consists of 196 amino acids after removal of the signal peptide and predicts a molecular mass of 22 kDa. It migrates as an approximately 30 kDa protein due to glycosylation by SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 97 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name | FCGR2A Fc fragment of IgG, low affinity IIa, receptor (CD32) [ Homo sapiens ] |
Official Symbol | FCGR2A |
Synonyms | FCGR2A; CD32; CD32A; CDw32; IGFR2; fcRII-a; fc-gamma-RIIa; FCG2; FcGR; FCGR2; FCGR2A1; MGC23887; MGC30032; |
Gene ID | 2212 |
mRNA Refseq | NM_001136219.1 |
Protein Refseq | NP_001129691.1 |
MIM | 146790 |
UniProt ID | P12318 |
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Not For Human Consumption!
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