Active Recombinant Human FBLN5 Protein, His-tagged

• Cat.No.: FBLN5-568H
• Product Overview: Recombinant Human FBLN5 (Accession # NP_006320) Gln24-Phe448, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Tag: His
• Predicted N Terminal: No results obtained: Gln24 predicted
• Form: Lyophilized from a 0.2 µm filtered solution in PBS and EDTA.
• Bio-activity: Measured by the ability of the immobilized protein to enhance the adhesion of HUVEC human umbilical vein endothelial cells.When 5 x 10^4 cells per well are added to rhFibulin-5 coated plate, cell adhesion is enhanced in a dose dependent manner after 60 minutes at 37 °C. ED50 for this effect is typically 0.1 - 0.4 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human FBLN5, His-tagged has a calculated MW of 48.6 kDa. In SDS-PAGE migrates as 60-66 kDa, reducing conditions.
• Protein length: Gln24-Phe448
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: FBLN5 fibulin 5 [ Homo sapiens ]
• Official Symbol: FBLN5
• Synonyms: FBLN5; fibulin 5
• Gene ID: 10433