Active Recombinant Human FBLN2 protein, HA-tagged

• Cat.No.: FBLN2-01H
• Product Overview: Human embryonic kidney cell, HEK293-derived human Fibulin 2 protein Ala28-Leu1184 (Thr854Ala), with an N-terminal HA tag.

Specification

• Description: This gene encodes an extracellular matrix protein, which belongs to the fibulin family. This protein binds various extracellular ligands and calcium. It may play a role during organ development, in particular, during the differentiation of heart, skeletal and neuronal structures. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Source: HEK293
• Species: Human
• Tag: HA
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of HFL1 human fetal lung fibroblast cells. The ED50 for this effect is 0.15-0.9 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 125 kDa
  SDS-PAGE: 150-175 kDa, reducing conditions
• N-terminal Sequence Analysis: Tyr (of HA tag)
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: > 90%
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
• Protein length: Ala28-Leu1184

Gene Information

• Gene Name: FBLN2 fibulin 2 [ Homo sapiens (human) ]
• Official Symbol: FBLN2
• Synonyms: FBLN2; FIBL-2; Fibulin 2; Fibulin-2
• Gene ID: 2199
• mRNA Refseq: NM_001004019
• Protein Refseq: NP_001004019
• MIM: 135821
• UniProt ID: P98095