Active Recombinant Human F7 Protein, His-tagged

• Cat.No.: F7-720H
• Product Overview: Recombinant Human F7 (Accession # NP_062562) Ala39-Pro444, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: Ala39-Pro444
• Predicted N Terminal: Ala39
• Form: Supplied as a 0.2 µm filtered solution in Sodium Acetate and NaCl.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC.The specific activity is >50 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human F7, His-tagged has a calculated MW of 46 kDa. In SDS-PAGE migrates as 60 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: F7 coagulation factor VII (serum prothrombin conversion accelerator) [ Homo sapiens ]
• Official Symbol: F7
• Synonyms: F7; coagulation factor VII (serum prothrombin conversion accelerator); coagulation factor VII; eptacog alfa; factor VII; FVII coagulation protein; SPCA; proconvertin; serum prothrombin conversion accelerator
• Gene ID: 2155
• mRNA Refseq: NM_000131
• Protein Refseq: NP_000122
• MIM: 613878
• UniProt ID: P08709
• Chromosome Location: 13q34