Active Recombinant Human F3 protein, His-tagged

• Cat.No.: F3-2643H
• Product Overview: Recombinant Human Coagulation factor III/CD142 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly34-Glu251) of human Coagulation Factor III/Tissue Factor/CD142 (Accession #NP_001984.1) fused with a 6×His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gly34-Glu251
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human CD142 at 1 μg/mL (100 μL/well) can bind Human Tissue Factor Rabbit mAb with a linear range of 0.98-61.71 ng/mL.
  2.Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC50 is <24.0 μg/mL.
• Molecular Mass: 37-45 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: > 95% by SDS-PAGE.
• Storage: Store the lyophilized protein at -20°C to -80 °C for long term.
  After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Gene Information

• Gene Name: F3 coagulation factor III (thromboplastin, tissue factor) [ Homo sapiens ]
• Official Symbol: F3
• Synonyms: F3; coagulation factor III (thromboplastin, tissue factor); tissue factor; CD142; TF; TFA; FLJ17960
• Gene ID: 2152
• mRNA Refseq: NM_001178096
• Protein Refseq: NP_001171567
• MIM: 134390
• UniProt ID: P13726