Active Recombinant Human ERBB3(Met1-Thr643), mFc-tagged

• Cat.No.: ERBB3-43H
• Product Overview: Active Recombinant Human ERBB3(Met1-Thr643)(P21860), fused with C-terminal mFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: mFc
• Protein length: 20-643 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human ErbB3-mFc at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1, The EC50 of biotinylated human NRG1 is 0.58-1.25 μg/ml.
• Molecular Mass: The recombinant human ErbB3/mFc is a disulfide-linked homodimer. The reduced monomer comprises 858 amino acids and has a predicted molecular mass of 95 kDa. The apparent molecular mass of the protein is approximately 119-129 in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ERBB3 v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) [ Homo sapiens ]
• Official Symbol: ERBB3
• Synonyms: ERBB3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian); LCCS2, lethal congenital contracture syndrome 2; receptor tyrosine-protein kinase erbB-3; HER3; proto-oncogene-like protein c-ErbB-3; tyrosine kinase-type cell surface receptor HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3; MGC88033
• Gene ID: 2065
• mRNA Refseq: NM_001005915
• Protein Refseq: NP_001005915
• MIM: 190151
• UniProt ID: P21860