Active Recombinant Human EPHB4 protein, His&GST-tagged

• Cat.No.: EPHB4-7278H
• Product Overview: Recombinant Human EPHB4 protein(P54760)(Leu563-Tyr987), fused with His&GST tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: Leu563-Tyr987
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 3mM DTT, 10% glyPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: The specific activity was determined to be 47 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
• Molecular Mass: The recombinant human EPHB4/GST chimera consists of 662 amino acids and has a calculated molecular mass of 75.2 kDa. The recombinant protein migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPHB4 EPH receptor B4 [ Homo sapiens ]
• Official Symbol: EPHB4
• Synonyms: EPHB4; EPH receptor B4; EphB4 , HTK; ephrin type-B receptor 4; Tyro11; ephrin receptor EphB4; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; hepatoma transmembrane kinase; tyrosine-protein kinase TYRO11; tyrosine-protein kinase receptor HTK; HTK; MYK1; TYRO11
• Gene ID: 2050
• mRNA Refseq: NM_004444
• Protein Refseq: NP_004435
• MIM: 600011
• UniProt ID: P54760