Active Recombinant Human EPHB3 Protein (Leu38-Ala550), Fc tagged

• Cat.No.: EPHB3-30H
• Product Overview: Recombinant human EPHB3 protein (Leu38-Ala550) with Fc tag was expressed in Mouse myeloma cell line.

Specification

• Species: Human
• Source: NS0
• Tag: Fc
• Protein Length: Leu38-Ala550
• Description: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. This gene encodes a receptor for ephrin-B family members.
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human EphB3 Fc Chimera at 2 μg/mL (100 μL/well) can bind recombinant mouse Ephrin-B1 Fc Chimera with a linear range of 0.04-2.5 ng/mL
• Molecular Mass: Predicted Molecular Mass: 82.7 kDa (monomer)
  SDS-PAGE: 90-100 kDa under reducing conditions
• N-terminal Sequence Analysis: Leu38
• Endotoxin: < 0.01 EU/μg of the protein by the LAL method.
• Purity: > 90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: EPHB3 EPH receptor B3 [ Homo sapiens (human) ]
• Official Symbol: EPHB3
• Synonyms: EPHB3; EPH receptor B3; EphB3, ETK2; ephrin type-B receptor 3; Hek2; Tyro6; EK2; EPH-like kinase 2; embryonic kinase 2; human embryo kinase 2; EPH-like tyrosine kinase 2; EPH-like tyrosine kinase-2; tyrosine-protein kinase TYRO6; ETK2; HEK2; TYRO6
• Gene ID: 2049
• mRNA Refseq: NM_004443
• Protein Refseq: NP_004434
• MIM: 601839
• UniProt ID: P54753