Active Recombinant Human EPHA5 protein, His-tagged

• Cat.No.: EPHA5-365H
• Product Overview: Recombinant Human EPHA5 protein(P54756-1)(Pro 25 - Pro 573) is expressed from HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Pro 25 - Pro 573
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Human Ephrin-A3, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human EphA5, His Tag with an affinity constant of 0.576 μM as determined in a SPR assay.
• Molecular Mass: The protein has a calculated MW of 62.7 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
  >95% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPHA5 EPH receptor A5 [ Homo sapiens ]
• Official Symbol: EPHA5
• Synonyms: EPHA5; EPH receptor A5; EphA5; ephrin type-A receptor 5; CEK7; EHK1; Hek7; TYRO4; EK7; EHK-1; EPH-like kinase 7; EPH homology kinase 1; Eph homology kinase-1; brain-specific kinase; receptor protein-tyrosine kinase HEK7; tyrosine-protein kinase receptor EHK-1; HEK7
• Gene ID: 2044
• mRNA Refseq: NM_004439
• Protein Refseq: NP_004430
• MIM: 600004
• UniProt ID: P54756