Active Recombinant Human EPHA4 protein(Met1-Thr547), His-tagged

• Cat.No.: EPHA4-3204H
• Product Overview: Recombinant Human EPHA4 (NP_004429.1)(Met 1-Thr 547) was expressed in HEK293, with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: Met1-Thr547
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind biotinylated Human EphrinA5-his in functional ELISA.
• Molecular Mass: The recombinant human EPHA4 consists of 539 amino acids and predictes a molecular mass of 60 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EPHA4 is approximately 60-70 kDa due to high levels of glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPHA4 EPH receptor A4 [ Homo sapiens ]
• Official Symbol: EPHA4
• Synonyms: EPHA4; EPH receptor A4; EphA4 , TYRO1; ephrin type-A receptor 4; Hek8; EK8; EPH-like kinase 8; TYRO1 protein tyrosine kinase; tyrosine-protein kinase TYRO1; tyrosine-protein kinase receptor SEK; receptor protein-tyrosine kinase HEK8; SEK; HEK8; TYRO1
• Gene ID: 2043
• mRNA Refseq: NM_004438
• Protein Refseq: NP_004429
• MIM: 602188
• UniProt ID: P54764