Active Recombinant Human EPHA3 protein(Met1-Gln541), His-tagged

• Cat.No.: EPHA3-4236H
• Product Overview: Recombinant Human EPHA3 (NP_005224.2) (Met1-Gln541) was expressed in HEK293, with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: Met1-Gln541
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized human EPHA3-His at 10 μg/ml (100 μl/well) can bind human EphrinA5-Fc, The EC50 of human EphrinA5-Fc is 6.2-14.6 ng/ml.
• Molecular Mass: The recombinant human EPHA3 consists of 532 amino acids and predicts a molecular mass of 60.3 KDa. It migrates as an approximately 65-70 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPHA3 EPH receptor A3 [ Homo sapiens ]
• Official Symbol: EPHA3
• Synonyms: EPHA3; EPH receptor A3; EphA3 , ETK, ETK1, TYRO4; ephrin type-A receptor 3; HEK; HEK4; EPH-like kinase 4; human embryo kinase 1; eph-like tyrosine kinase 1; TYRO4 protein tyrosine kinase; tyrosine-protein kinase receptor ETK1; EK4; ETK; ETK1; TYRO4
• Gene ID: 2042
• mRNA Refseq: NM_005233
• Protein Refseq: NP_005224
• MIM: 179611
• UniProt ID: P29320