Active Recombinant Rat Epha3 protein(Met1-His541), His-tagged

• Cat.No.: Epha3-552R
• Product Overview: Recombinant Rat EPHA3 (EDL75897.1) (Met1-His541) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Met1-His541
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized rat EPHA3-His at 10 μg/ml (100 μl /well) can bind rat EFNA5-Fc, The EC50 of rat EFNA5-Fc is 9-20ng/ml.
• Molecular Mass: The recombinant rat EPHA3 comprises 532 amino acids and predicts a molecular mass of 61.2 kDa. The apparent molecular mass of the recombinant protein is approximately 70.5 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Epha3 Eph receptor A3 [ Rattus norvegicus ]
• Official Symbol: Epha3
• Synonyms: EPHA3; Eph receptor A3; ephrin type-A receptor 3; EK4; rEK4; EPH-like kinase 4; tyrosine-protein kinase TYRO4; tyrosine-protein kinase receptor REK4
• Gene ID: 29210
• mRNA Refseq: NM_031564
• Protein Refseq: NP_113752