Active Recombinant Human EGFR protein(Met1-Lys375), hFc-tagged

• Cat.No.: EGFR-689H
• Product Overview: Recombinant Human EGFR (NP_001333870.1) (Met1-Lys375) was expressed in Insect Cells with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Fc
• ProteinLength: Met1-Lys375
• Form: Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Immobilized EGF Protein, Human, Recombinant (ECD), HPLC-verified at 2 μg/mL (100 μL/well) can bind EGFR Protein, Human, Recombinant (Isoform Viii, hFc Tag) in functional ELISA. 2. Immobilized Anti-EGFR(MK)-IgG1 Antibody (Cetuximab) at 2 μg/mL (100 μL/well) can bind EGFR Protein, Human, Recombinant (Isoform Viii, hFc Tag), the EC50 is 4-13 ng/mL.
• Molecular Mass: The recombinant human EGFR consists of 589 amino acids and predicts a molecular mass of 65.1 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533