Active Recombinant Human EGFR Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: EGFR-464HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human EGFR (Accession # CAA25240) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by its ability to bind rhEGF in a functional ELISA with an estimated KD < 8 nM.
• Molecular Mass: Recombinant Human EGFR, Fc Chimera has a calculated MW of 95.1 kDa (monomer). In SDS-PAGE migrates as 125-145 kDa, reducing conditions.
• N-terminal Sequence Analysis: Leu 25
• Purity: > 90 % by SDS-PAGE and analyzed by silver stain
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533