Active Recombinant Human EGF Protein, Pre-aliquoted

• Cat.No.: EGF-16H
• Product Overview: Recombinant Human EGF Protein, Asn971 - Arg1023, with an N-terminal Met, was expressed in E. coli.

Specification

• Description: This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
• Source: E. coli
• Species: Human
• Bio-activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 20-100 pg/mL.
• Molecular Mass: Predicted Molecular Mass:6 kDa
  SDS-PAGE: 6 kDa, reducing conditions
• Protein length: Asn971-Arg1023, with an N-terminal Met
• N-terminal Sequence Analysis: Met
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 97% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature as supplied.
  • 12 months from date of receipt at 2-8 centigrade as supplied.
  • 1 month at 2-8 centigrade under sterile conditions after reconstitution.
  • 3 months at -20 to -80 centigrade under sterile conditions after reconstitution.
• Reconstitution: For a stock solution, reconstitute at 500 μg/mL in sterile PBS, or simply roll Pre-aliquoted directly into cell culture medium for immediate use.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: EGF epidermal growth factor [ Homo sapiens (human) ]
• Official Symbol: EGF
• Synonyms: EGF; epidermal growth factor; URG; HOMG4; pro-epidermal growth factor; beta-urogastrone
• Gene ID: 1950
• mRNA Refseq: NM_001963
• Protein Refseq: NP_001954
• MIM: 131530
• UniProt ID: P01133