Acitve Recombinant Human EFNB2 protein(Met1-Ala229), His&hFc-tagged

• Cat.No.: EFNB2-1374H
• Product Overview: Recombinant Human EFNB2 (NP_004084.1)(Met 1-Ala 229) was expressed in HEK293, fused with a C-terminal Fc region of human IgG1 tag followed by a polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His&hFc
• Protein length: Met1-Ala229
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human EphB4-his at 2 μg/ml (100 μl/well) can bind Human EphrinB2 Fc chimera with a linear range of 1-25 ng/ml. Immobilized Human EphB4 at 2 μg/ml (100 μl/well) can bind Human EphrinB2 Fc chimera with a linear range of 1-125 ng/ml.
• Molecular Mass: The recombinant Human EFNB2/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 450 amino acids and predicts a molecular mass of 50.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EFNB2/Fc monomer is approximately 62 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EFNB2 ephrin-B2 [ Homo sapiens ]
• Official Symbol: EFNB2
• Synonyms: EFNB2; ephrin-B2; EPLG5; eph related receptor tyrosine kinase ligand 5; HTK ligand; Htk L; HTKL; LERK5; ligand of eph related kinase 5; MGC126226; MGC126227; MGC126228; LERK-5; ligand of eph-related kinase 5; eph-related receptor tyrosine kinase ligand 5; Htk-L
• Gene ID: 1948
• mRNA Refseq: NM_004093
• Protein Refseq: NP_004084
• MIM: 600527
• UniProt ID: P52799