Active Recombinant Human EDIL3 Protein, His-tagged

• Cat.No.: EDIL3-974H
• Product Overview: Recombinant Human EDIL3 (Accession # O43854) Val17-Glu480, fused with a C-terminal 6x His, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Val17, Asp24
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells.When 5 x 10^4 cells/well are added to Recombinant Human EDIL3 coated plates (5 µg/mL, 100 µL/well), >45% will adhere after 60 minutes at 37 °C.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human EDIL3, His-tagged has a calculated MW of 52 kDa. In SDS-PAGE migrates as 60-65 kDa, reducing conditions.
• Protein length: Val17-Glu480
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: EDIL3 EGF-like repeats and discoidin I-like domains 3 [ Homo sapiens ]
• Official Symbol: EDIL3
• Synonyms: EDIL3; EGF-like repeats and discoidin I-like domains 3; EGF-like repeat and discoidin I-like domain-containing protein 3; DEL1; integrin-binding protein DEL1; developmental endothelial locus-1; developmentally-regulated endothelial cell locus 1 protein; MGC26287
• Gene ID: 10085
• mRNA Refseq: NM_005711
• Protein Refseq: NP_005702
• MIM: 606018
• UniProt ID: O43854
• Chromosome Location: 5q14